Mass Spectrometry Laboratory

School Affiliated departments and LAS

Chemistry
College of Liberal Arts & Sciences

AB Voyager MALDI

(Acquired in 1998)

The Voyager is a high performance matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI-TOFMS) with a mass range of 500-300,000 Da. It is equipped with a nitrogen laser (337nm, 3 ns pulse, 20 Hz maximum firing rate.). It can operate in the linear and reflectron modes. The linear detector allows for better sensitivity of high mass molecules, while the reflectron detector provides high resolution, permitting isotopic separations up to 5,000 daltons. Mass measurement accuracy is typically 0.05-0.1% in the linear mode and 0.005-0.01% in the reflection mode. The Voyager is equipped with a collision gas cell and a timed-ion-selector for performing MS/MS employing the collision-induced dissociation/post-source-decay method (CID/PSD) for sequencing peptides and oligonucleotides.

The sensitivity of detection depends upon the ease with which each compound can be charged or protonated. The limit of detection is in the sub-picomolar range. We require high purity samples. As the molecular weight or impurities (including buffer adduct ions like sodium) increases, the pattern of the protein ions (protein envelope) becomes less distinct and the standard deviation for the calculated molecular weight is greater.

Data acquisition and processing is controlled by the Applied Biosystems software running on a PC. Output for Voyager is in the form of smoothed averaged data.

The Voyager is the primary instrument for matrix-assisted laser desorption/ionization (MALDI).

Due to its ease of operation, the Voyager is available for self use by graduate students and post-docs after training by MS facility staff. To receive training on the Voyager, contact Furong Sun at frs [at] illinois [dot] edu .

Applied Biosystems Voyager-DE STR
University of Illinois at Urbana-Champaign
Mass Spectrometry Laboratory
35 Noyes Laboratory
MC-712 Box 51-1
505 South Mathews Ave.
Urbana, IL 61801
Furong Sun
Director

tel: 217/333-2545
frs [at] illinois [dot] edu
Lab Hours: 24/7 access by key
Staff Available 8 am to 4:45 pm
Monday through Friday

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