Mass Spectrometry Laboratory

School Affiliated departments and LAS

College of Liberal Arts & Sciences

Automated Data Dependent Acquisition (DDA) — (HPLC/MS/MS)

HPLC/MS/MS can be done for both known and unknown compounds. For the automated MS/MS analysis of unknown compounds, several of our LC-MS systems have a powerful software control system to enable the instrument to perform data dependent acquisition (DDA), switching from the MS to MS/MS mode and then returning to the MS mode using data dependent criteria.

This method obviates the need to analyze the sample in MS mode to identify the target precursor ions and then re-run the sample in MS/MS mode to acquire the MS/MS data from each of these precursors. This is particularly valuable in the analysis of unknown samples using on-line chromatography where the target precursor ions and their retention times may be different for each sample.
University of Illinois at Urbana-Champaign
Mass Spectrometry Laboratory
35 Noyes Laboratory
MC-712 Box 51-1
505 South Mathews Ave.
Urbana, IL 61801
Furong Sun

tel: 217/333-2545
frs [at] illinois [dot] edu
Lab Hours: 24/7 access by key
Staff Available 8 am to 4:45 pm
Monday through Friday

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