Mass Spectrometry Laboratory

School Affiliated departments and LAS

Chemistry
College of Liberal Arts & Sciences

Sample Submission Guidelines

  1. Routine accurate mass measurements can be done by EI, CI, ESI, and FAB
    • The upper mass range for HR ESI is ~ 2000 Da
    • The upper mass range for HR FAB is ~ 2200 Da
    • The upper mass range for HR EI/CI is ~ 800 Da
  2. The upper mass range for LR FD/FAB is ~ 5000 Da
  3. Air sensitive samples can be run by EI, CI, FI, FD, ESI, and LIFDI with advanced notice. The user will have to provide dry solvent(s) for ESI and FD.
  4. Conductive polymers and carbon nanotubes as MALDI matrixes cause MALDI instruments to malfunction. Conductive polymer MALDI matrixes should not be run on MALDI without specific approval of the lab director and carbon nanotubes matrixes should not be used.
  5. MALDI samples on glass chips or samples need a special adapter for the MALDI target frame. They must be approved by the lab director before analysis each time.
  6. Instrument maintenances can only be performed by the lab staff. Modifications for any instrument are not allowed.
  7. For LCMS samples, a protocol either developed on your own or from the literature for the similar compounds is preferred. Mass Spectrometry Facility’s general purpose protocol is available but your samples should not cause any damage and> should not stick on the columns if a Mass Spectrometry Facility column is needed.
  8. On the chart http://scs.illinois.edu/massSpec/usage/, there are points where a decision leads to a split recommendation, which indicates that the sample should generally work in both techniques. Guidelines for these samples are:
    • ESI/FAB samples should be run in ESI, unless they are known or expected to work better in FAB.
    • CI/FI samples should be run in CI if HR is needed

March 10, 2017

University of Illinois at Urbana-Champaign
Mass Spectrometry Laboratory
35 Noyes Laboratory
MC-712 Box 51-1
505 South Mathews Ave.
Urbana, IL 61801
Furong Sun
Director

tel: 217/333-2545
frs [at] illinois [dot] edu
Lab Hours: 24/7 access by key
Staff Available 8 a.m. to 5 p.m.
Monday through Friday

Please send Web site comments and suggestions to:
scs_web [at] scs [dot] illinois [dot] edu